How to correctly reconstitute Botox (botulinum toxin type A)?

The dilution of botulinum toxin depends on the brand, purpose of use, and concentration.
Here are the general principles and dilution guidelines for popular products.

📌 Key Rules for Botox Dilution

• • ✔ Use sterile 0.9% saline solution (sodium chloride).
  • ✔ Do not shake the vial! – Gently inject the solution along the vial’s wall.
  • ✔ Store the reconstituted solution in the refrigerator (2–8°C) and use within 24 hours (preferably immediately after dilution).
  • ✔ Dilution depends on the dosage and injection area – different zones require different concentrations.

 

📌 Botox Dilution – Standard Protocol

1. Dilution Ratio (Units per Milliliter)

Volume of Diluent	Concentration (Units/0.1 mL)
1 mL saline	10 units
2 mL saline	5 units
2.5 mL saline	4 units
4 mL saline	2.5 units

☝ Example: If a vial contains 100 units of botulinum toxin, the dilution will be:

•1 mL saline = 100 U/mL

•2.5 mL saline = 40 U/mL

•4 mL saline = 25 U/mL

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2. Step-by-Step Dilution Process

1. Prepare sterile 0.9% sodium chloride (saline solution).
2. Slowly inject the solution through the vial’s rubber stopper along the inner wall (DO NOT shake!).
3. Let it sit for 1–2 minutes for full dissolution.
4. Draw the solution into a syringe – ready for use.

📌 Botox Dilution by Brand

Product	Saline Volume (per 100 U)	Concentration (U/0.1 mL)
Botox (Allergan)	2.5 mL	4 U/0.1 mL
Dysport	2.5 mL	10 U/0.1 mL
Nabota (Daewoong)	2.5 mL	4 U/0.1 mL
Botulax	2.5 mL	4 U/0.1 mL
Neuronox	2.5 mL	4 U/0.1 mL

☝ Dysport is diluted differently:
It is diluted more (typically, 2.5 mL of saline results in 10 U/0.1 mL, which is equivalent to 4 U/0.1 mL of Botox/Nabota).

📌 How much Botox to inject in different areas?

Area	Number of Units (for the entire area)
Forehead	8–20 U
Glabella (between the eyebrows)	10–25 U
Crow’s feet	6–15 U
Brow lift	4–8 U
Nostrils (lateral contraction)	2–6 U
Masseter muscles (bruxism, V-line)	25–50 U
Chin (orange peel)	4–8 U
Neck (Venus rings)	20–50 U
Armpits (hyperhidrosis)	50–100 U

⚠️ Dosage may vary depending on the individual characteristics of the patient!

📌 Important Recommendations

✔ Needles – 30G 0.3 mm for precise injections.

✔ Injection depth – depends on the area (superficial for wrinkles, deeper for masseter muscles).

✔ Onset of action – the effect appears within 3–7 days, with the maximum effect seen after 2 weeks.

✔ Side effects – possible swelling, bruising, and asymmetry with improper injection.

📌 Conclusion: Botox dilution depends on the brand, area of application, and desired concentration. If you have a specific product, I can provide the exact dilution protocol for it. 😊